Invasive species trigger rapid evolution for lizards in Florida

A green anole, clinging to a palm frond with nicely silhouetted toepads. Yoel Stuart, Author provided

Invasive species colonize and spread widely in places where they are not normally found. Invasives often affect native species by eating them, out-competing them and introducing unfamiliar parasites and pathogens. For example, the invasive Kudzu plant, native to southeast Asia, overgrows seemingly anything in its path in the southeast US.

Invasive Kudzu vines make themselves at home. Gsmith, CC BY-SA

Natural selection wrought by invasive species can often be strong and natives will either go extinct or adapt. During adaptation, selection will favor those individuals with characteristics that best allow them to survive and reproduce in the face of the invader. The offspring of the survivors will inherit their parents’ beneficial traits, and the population will evolve.

Invasive brown anole male, perched and extending a flap of skin called the dewlap. It uses the dewlap to communicate during territorial and mating interactions. Dr. Todd Campbell

In the 1950s, the brown anole lizard, Anolis sagrei, arrived in south Florida from Cuba. It quickly boomed to become arguably Florida’s most abundant vertebrate by biomass. The effects of this invasion might not be very noticeable to humans (though their cats have paid rapt attention). But the brown anole certainly makes an impression on Florida’s only native anole species, the green anole, Anolis carolinensis.

Native green anole male, perched and dewlapping. Ambika Kamath

This is because the green and the brown anoles enjoy similar lifestyles. They are similarly sized, both active during the day and both defend territories. They eat similar food – mostly insects and spiders – and use similar habitat – the ground and lower parts of trees and bushes. Because of these similarities, we expect the invasive brown anole to impose strong natural selection on the native green.

Thus, my colleagues and I asked: how is the green anole responding to the brown anole invasion?

50-year-old, man-made, dredge spoil islands in Mosquito Lagoon, FL. Note the houseboat in the foreground of the image for scale. The islands are about a football field in size. Todd Campbell

Researchers before us had observed that green anoles living with brown anoles tend to live higher up in the trees, presumably to escape competition for food and space. Definitive evidence required an experiment. So we got to work on small, man-made islands near Cape Canaveral.

In 1995, we introduced the brown anole to three islands that – until then – had only green anoles. Within a few months, the green anole moved up into the trees and stayed there: clear experimental evidence that the brown anole affects the green by changing green anole behavior.

On the introduction islands (filled shapes), the green anole increased its perch height within months. Stuart et al. 2014. Science 346, 463-466

Fifteen years later, my colleagues and I wondered whether the green anoles had adapted anatomically to their new life up in the trees. We were specifically interested in toepads on their feet; other anole species that live high in trees tend to have large toepads, the better to grasp smoother, narrower branches higher up.

We would have liked to study toepad evolution in the same populations we’d looked at earlier. But the original control islands, with only green anoles, had been invaded by the brown anole by the time we revisited them in 2010. So instead, we chose five large islands that had just green anoles, the only such islands left in the lagoon. We compared their green anoles to the green anoles on six large islands that had been naturally invaded by the brown anole. We did know that the brown anoles had hit the scene sometime between 1995 and 2010 because we had surveyed the islands in 1995 and found them free of brown anoles at that time.

Yoel Stuart attempting to snare a lizard in a buttonwood tree. Adam Algar, Author provided

Using little lassos on the end of fishing poles, we captured green anoles. At our field station, we anesthetized them and took digital scans of their toepads. Then we let the lizards wake up and recover overnight, and we released them the next day at the spot we caught them. We often wondered whether their friends believed their abduction stories.

Anesthetized green anole on the flatbed scanner, having its toepads imaged. Maureen Stimola, Author provided
This graph shows an increase in toepad area in green anoles caught on invaded islands. The units are odd because of the need to correct for body size when comparing toepad area. Stuart et al. 2014. Science, 346: 463-466

We found that on the invaded islands, green anoles evolved larger toepads. It took only 20 generations – less than 15 years – for the toepads to increase by about 5%. That may not sound like much, but that’s a rapid evolutionary pace. For comparison, if the American population was evolving to be taller at that speed, the average height of American men would be seven inches taller in 20 generations. Our findings further support the notion that when natural selection is strong, evolution can proceed quite quickly.

Right foot of a green anole male. Note the expanded scales on the ends of the toes. These have special hairs on them that allow anoles to cling to surfaces using van der Waals forces. Yoel Stuart

Why did selection favor larger toepads? Like geckos, anoles’ toes have specialized scales with fine hairs on them that cling to surfaces. Anoles with larger toepads are better at clinging. We think that the green anoles were under selection to get better at maneuvering on narrow, flexible and slippery twigs and leaves high in trees. Thus, green anole hatchlings that were born with larger toepads were better able to grow, survive, and reproduce. They passed their genetic traits on to the next generation.

In this case, it appears that the green anole has been able to adapt to coexist with the brown anole. It will not be going locally extinct any time soon. We’ll just have to look up to find it.

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